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 ORIGINAL ARTICLE
Year : 2006  |  Volume : 43  |  Issue : 4  |  Page : 163-168

Expression of cyclooxygenase-2 and 12-lipoxygenase in human breast cancer and their relationship with HER-2/neu and hormonal receptors: Impact on prognosis and therapy


1 Biochemistry and Molecular Biology Unit, Departments of Cancer Biology, NCI, Cairo University, Egypt
2 Biochemistry and Molecular Biology Unit, Departments of Oncology, NCI, Cairo University, Egypt
3 Biochemistry and Molecular Biology Unit, Departments of Surgical Pathology, NCI, Cairo University, Egypt

Correspondence Address:
A M Mohammad
Biochemistry and Molecular Biology Unit, Departments of Cancer Biology, NCI, Cairo University
Egypt
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0019-509X.29421

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Background: A number of studies have shown over-expression of cox-2 in breast cancer. Also it has been recorded that human breast cancer expresses high level of cox-2 and 12-lipoxygenase which may be beneficial in future therapy plan for those patients. Aims:The present study aims to examine the level of transcripts of cox-2 and 12-lipoxygenase in Egyptian breast cancer patients and to compare between the expressions of both enzymes and TNM staging, hormone receptors status (including estrogen and progesterone) and HER2/neu expression. Materials and Methods:Total cellular RNA was extracted from 64 frozen tissue samples of breast carcinoma and their corresponding normal adjacent tissues. Cox-2 and 12-lipooxygenase expressions were detected using RT-PCR. Hormonal receptors as well as HER2/neu were detected immuno-histochemically for each patient. Results: About 47 and 62.5% of carcinoma samples showed over-expression of cox-2 and 12-lipooxygenase respectively as compared to their corresponding normal tissues. The results revealed that cox-2 significantly associated with TNM staging ( P =0.0047) and hormonal receptors status ( P = 0.0201). The relationship between cox-2 and HER2/neu expression was close to a significant value ( P =0.0747). 12-lipooxygenase showed only significant association with TNM staging ( P =0.0076). Neither hormonal receptors nor HER2/neu showed significant association with this enzyme. Conclusion: Elevated levels of cox-2 and 12-lipoxygenase expression were detected in human breast cancer. Also, the results revealed that cox-2 and 12-lipooxygenase mRNA expressions are associated with TNM staging in human breast cancer. Furthermore, there is an inverse association between cox-2 expression and hormonal receptor status. This observation may drive us to the possible role of those two enzymes in determining the plan of therapy of breast cancer patients.






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