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 ORIGINAL ARTICLE
Year : 2008  |  Volume : 45  |  Issue : 1  |  Page : 8-12

Relationship between genetic polymorphism of glutathione S-transferase-p1 and p53 protein accumulation in Iranian esophageal squamous cell carcinoma patients


1 Department of Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, P. O. Box: 14115-331, Tehran, I. R, Iran
2 National Research Center for Genetic Engineering and Biotechnology, Tehran, I. R, Iran
3 Department of Biochemistry and Genetics, Shaheed Beheshti Univerity of Medical Sciences, Tehran, IR, Iran
4 Department of Pathology, Shariati Hospital, Tehran, I.R, Iran
5 Department of Pathology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Correspondence Address:
A Allameh
Department of Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, P. O. Box: 14115-331, Tehran, I. R
Iran
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0019-509X.40640

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Background: It has been reported that the activity of glutathione S-transferase (GST) is over-expressed in plasma and esophagus biopsies in Iranian patients suffering from esophageal squamous cell carcinoma (SCC). The aim of this study was to find out the frequency of GST-P genotypes in these patients. Moreover, the association of GST-P genotypes with p53 protein accumulation in esophageal epithelium was investigated. Materials and Methods: DNA isolated from paraffin-embedded tissue biopsies from patients suffering from esophageal SCC (n = 56) were collected. polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using Alw261 enzyme was applied to determine GST-P genotypes (Ile 105 Val). All the samples were also subjected to immunohistochemistry (IHC) for p53. Results: The frequency of GST-P genotypes in Iranian esophagus SCC patients for Ile/Ile, Ile/Val and Val/Val was 73.2, 21.5 and 5.3%. There was no association between GST-P polymorphism and p53 accumulation in esophageal epithelial cells. Conclusions: The frequency of GST-P polymorphism was not associated with p53 protein accumulation in esophagus epithelium. The frequency of polymorphic variants of GST-P, Ile/Ile, Ile/Val and Val/Val in SCC patients may suggest that Ile to Val substitution in GST-P gene dose not represent susceptibility to SCC in high-risk Iranian population.






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