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Year : 2015  |  Volume : 52  |  Issue : 7  |  Page : 172--175

Receptor for activated protein kinase C 1 suppresses gastric tumor progression through nuclear factor-kB pathway

X Yong-zheng, M Wan-li, M Ji-ming, R Xue-qun 
 Department of General Surgery, Huaihe Hospital of Henan University, Kaifeng 475000, Henan Province, PR China

Correspondence Address:
R Xue-qun
Department of General Surgery, Huaihe Hospital of Henan University, Kaifeng 475000, Henan Province
PR China

OBJECTIVE: Nuclear factor-kB (NF-kB) activity is crucial for survival and proliferation of many kinds of malignancies, including gastric cancer (GC). The receptor for activated protein kinase C 1 (RACK1) is known to regulate tumor development, whereas the underlined mechanism has not been described clearly. MATERIALS AND METHODS: We analyzed expression of RACK1 in paired human GC samples by both real-time polymerase chain reaction (PCR) and western blot. Effects of RACK inhibition with small interfering RNA or its overexpression in cultured GC cell lines were evaluated in cell viabilities. NF-kB signaling was investigated using luciferase reporter assay and real-time PCR. RESULTS: RACK1 was significantly decreased in GC samples. Knockdown of RACK elevated GC cell viabilities, whereas overexpression of RACK1 suppressed tumorigenesis of GC cells. Importantly, NF-kB signaling was enhanced after RACK1 expression was inhibited, suggesting the negative regulation of the pro-oncogenic NF-kB activity by RACK1 might contribute to its tumor suppressor role in GC cells. CONCLUSION: Our results support that RACK1 suppresses gastric tumor progression through the NF-kB signaling pathway.


How to cite this article:
Yong-zheng X, Wan-li M, Ji-ming M, Xue-qun R. Receptor for activated protein kinase C 1 suppresses gastric tumor progression through nuclear factor-kB pathway.Indian J Cancer 2015;52:172-175


How to cite this URL:
Yong-zheng X, Wan-li M, Ji-ming M, Xue-qun R. Receptor for activated protein kinase C 1 suppresses gastric tumor progression through nuclear factor-kB pathway. Indian J Cancer [serial online] 2015 [cited 2020 Sep 23 ];52:172-175
Available from: http://www.indianjcancer.com/article.asp?issn=0019-509X;year=2015;volume=52;issue=7;spage=172;epage=175;aulast=Yong-zheng;type=0