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Figure 2 :Genomic polymerase chain reaction (PCR) was performed for ErbB-2 and GAPDH as described in methods and the PCR products were resolved in a 2.0% agarose gel. Representative gels for the same are shown. Q-value (fold amplifi cation) was calculated as ratio of ErbB-2/GAPDH levels for tumor sample over average of ratio of ErbB-2/GAPDH levels for normal samples. A cut-off value of 2.0 was used to identify the ErbB-2 overexpressing samples. (A) Representative agarose gel for ErbB-2 and GAPDH for normal samples (N1N10) (B) Representative agarose gel for ErbB-2 and GAPDH for tumor samples (T13T22) (C) Q-values for ErbB-2 of all 55 tumor samples and 16 normals plotted in a scatter plot

Figure 2 :Genomic polymerase chain reaction (PCR) was performed for ErbB-2 and GAPDH as described in methods and the PCR products were resolved in a 2.0% agarose gel. Representative gels for the same are shown. Q-value (fold amplifi cation) was calculated as ratio of ErbB-2/GAPDH levels for tumor sample over average of ratio of ErbB-2/GAPDH levels for normal samples. A cut-off value of 2.0 was used to identify the ErbB-2 overexpressing samples. (A) Representative agarose gel for ErbB-2 and GAPDH for normal
samples (N1N10) (B) Representative agarose gel for ErbB-2 and GAPDH for tumor samples (T13T22) (C) Q-values for ErbB-2 of all 55 tumor samples and 16 normals plotted in a scatter plot