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Figure 3: Effects of sphingosine kinase 1 (Sphk1) over-.expression in MCF-.10A. (a) Real time-.polymerase chain reaction detection of Sphk1 and E-.cadherin (E-.cad) expression; (b) western-.blot detection of Sphk1 and E-.cad expression; (c) immunohistochemical detection of Sphk1 expression; (d) effects on cell proliferation; (e and f) effects on cell invasion. (1) MCF-.10A; (2) MCF-.10A-.empty plasmid; (3) MCF-.10A-.Sphk1

Figure 3: Effects of sphingosine kinase 1 (Sphk1) over-.expression in MCF-.10A. (a) Real time-.polymerase chain reaction detection of Sphk1 and E-.cadherin (E-.cad) expression; (b) western-.blot detection of Sphk1 and E-.cad expression; (c) immunohistochemical detection of Sphk1 expression; (d) effects on cell proliferation; (e and f) effects on cell invasion. (1) MCF-.10A; (2) MCF-.10A-.empty plasmid; (3) MCF-.10A-.Sphk1