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Figure 3: miR - 338 - 3p inhibits epithelial - mesenchymal transition by target zinc finger E - box - binding protein 2 in A549 cells. (a) Protein levels of epithelial-mesenchymal transition markers, E-cadherin, N-cadherin, and vimentin were detected by Western blot in A549 cells transfected with miR-338-3p mimic. (b) The schematic diagram shows the luciferase reporter of miR - 338 - 3p binding site on the wild - type (blue) and mutant (red) zinc finger E - box - binding protein 2 3′ -untranslated region. (c) Relative Dual-luciferase activity analyses. The pmirGLO-ZEB2-3′ UTR-WT or pmirGLO-ZEB2-3′ UTR-MUT was transfected into A549 cells with miR - 338 - 3p mimic or its control plasmid. Renilla luciferase activity was used as internal controls. **P < 0.01

Figure 3: miR - 338 - 3p inhibits epithelial - mesenchymal transition by target zinc finger E - box - binding protein 2 in A549 cells. (a) Protein levels of epithelial-mesenchymal transition markers, E-cadherin, N-cadherin, and vimentin were detected by Western blot in A549 cells transfected with miR-338-3p mimic. (b) The schematic diagram shows the luciferase reporter of miR - 338 - 3p binding site on the wild - type (blue) and mutant (red) zinc finger E - box - binding protein 2 3′ -untranslated region. (c) Relative Dual-luciferase activity analyses. The pmirGLO-ZEB2-3′ UTR-WT or pmirGLO-ZEB2-3′ UTR-MUT was transfected into A549 cells with miR - 338 - 3p mimic or its control plasmid. Renilla luciferase activity was used as internal controls. **<i>P </i>< 0.01