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Figure 2: MicroRNA-185 inhibits cell proliferation of colorectal cancer cells. (a) Left panel: LoVo cells were transfected with microRNA-185 mimics duplexes or control duplexes. In 48 h, the cells were harvest for testing the overexpression effect. Right panel: LoVo cells were transfected with microRNA-185 mimics duplexes or control duplexes. In 24 h, cells were trypsinized and seeded into four 96-well plates at a density of 1.5 103 cells for cell counting kit-8 assay. (b) Left panel: SW480 cells were transfected with microRNA-185 inhibitor oligoes or control oligoes. In 48 h, the cells were harvest for testing the knockdown effect. Right panel: SW480 cells were transfected with microRNA-185 inhibitor oligoes or control oligoes. In 24 h, cells were trypsinized and seeded into four 96-well plates at a density of 1.5 103 cells for cell counting kit-8 assay

Figure 2: MicroRNA-185 inhibits cell proliferation of colorectal cancer cells. (a) Left panel: LoVo cells were transfected with microRNA-185 mimics duplexes or control duplexes. In 48 h, the cells were harvest for testing the overexpression effect. Right panel: LoVo cells were transfected with microRNA-185 mimics duplexes or control duplexes. In 24 h, cells were trypsinized and seeded into four 96-well plates at a density of 1.5  10<sup>3</sup> cells for cell counting kit-8 assay. (b) Left panel: SW480 cells were transfected with microRNA-185 inhibitor oligoes or control oligoes. In 48 h, the cells were harvest for testing the knockdown effect. Right panel: SW480 cells were transfected with microRNA-185 inhibitor oligoes or control oligoes. In 24 h, cells were trypsinized and seeded into four 96-well plates at a density of 1.5  10<sup>3</sup> cells for cell counting kit-8 assay