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Figure 3: MicroRNA-185 inhibits Wnt/ƒÀ -catenin signaling in colorectal cancer cells. (a) TOPFlash reporter activity in LoVo and SW480 cells. Cells were co-transfected with TOPFlash as well as either negative control or microRNA-185 mimics/inhibitor. TOPFlash transcription was determined by the TOPFlash luciferase activity. Reporter activities were normalized to internal control Renilla. (b) Reverse transcriptional polymerase chain reaction and immunoblotting to examine expression for two Wnt/ƒÀ -catenin signaling pathway target proteins, MYC and CYCLIND1 in LoVo and SW480 cells

Figure 3: MicroRNA-185 inhibits Wnt/ƒÀ -catenin signaling in colorectal cancer cells. (a) TOPFlash reporter activity in LoVo and SW480 cells. Cells were co-transfected with TOPFlash as well as either negative control or microRNA-185 mimics/inhibitor. TOPFlash transcription was determined by the TOPFlash luciferase activity. Reporter activities were normalized to internal control Renilla. (b) Reverse transcriptional polymerase chain reaction and immunoblotting to examine expression for two Wnt/ƒÀ -catenin signaling pathway target proteins, MYC and CYCLIND1 in LoVo and SW480 cells