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Figure 3: MicroRNA-618 inhibits TPC-1 cells viability partly through Akt. (a) TPC-1 cells were transfected with microRNA-618 mimics or negative controls and subjected to quantitative real-time polymerase chain reaction analysis. (b) The expression of p-PI3K and p-Akt was analyzed by Western blotting. GAPDH was used as an internal control. (c) Reverse experiment of co-transfected microRNA-618 mimics with myc-Akt-ca plasmid in TPC-1 cells by 3 - (4,5 - dimethylthiazol - 2 - yl) - 2,5 - diphenyl - tetrazoliumbromide assay. *P < 0.05. **P < 0.001

Figure 3: MicroRNA-618 inhibits TPC-1 cells viability partly through Akt. (a) TPC-1 cells were transfected with microRNA-618 mimics or negative controls and subjected to quantitative real-time polymerase chain reaction analysis. (b) The expression of p-PI3K and p-Akt was analyzed by Western blotting. GAPDH was used as an internal control. (c) Reverse experiment of co-transfected microRNA-618 mimics with myc-Akt-ca plasmid in TPC-1 cells by 3 - (4,5 - dimethylthiazol - 2 - yl) - 2,5 - diphenyl - tetrazoliumbromide assay. *<i>P</i> < 0.05. **<i>P</i> < 0.001